The answers that I got are 1 Yellow 2 Blue 3 Red 4 Yellow If

The answers that I got are 1. Yellow 2. Blue 3. Red 4. Yellow. If I am not correct, then explain why. Thanks!

D. Consider the following primed template used in DNA sequencing: Template strand Primer 5\' TCGGCATGCAGT3\' If each of the dideoxy nucleotides used in DNA sequencing has a different fluorescent dye attached (ddA - RED; ddC- YELLOW; ddG- GREEN; ddT - BLUE) i) What will be the color of the first four sequencing fragments detected by the automated DNA sequencer? (4)

Solution

This method of DNA sequence determination is called as the termination method. These dideoxynucleotides can not add any more nucleotides and the chain terminates with their incorporation. Thus, the sequences reads the complementary strand to the original sequence. Your answer is correct. This will be the actual order of the first four nucleotides.