The restriction enzymes XhoI and SalI cut their specific seq

The restriction enzymes XhoI and SalI cut their specific sequences as shown below:

Part A: Can the sticky ends created by XhoI and SalI sites be ligated? Yes or No?

Part B: Can the resulting sequences be cleaved by XhoI? Yes or No?

Part C: Can the resulting sequences be cleaved by SalI? Yes or No?

XhoI	5\'-C	TCGAG-3\'
	3\'-GAGCT	C-5\'
SalI	5\'-G	TCGAC-3\'
	3\'-CAGCT	G-5\'

XhoI and SalI generate compatible sticky ends and therefore they can be ligated together. However the recombinant site that is generated can not be digested by either enzyme afterwards.

PART A YES

PARTB NO

PART C NO

The restriction enzymes XhoI and SalI cut their specific seq

Solution

Get Help Now

Submit a Take Down Notice