A write the amino acid sequence for the DNA strand below ass

A. write the amino acid sequence for the DNA strand below. assume this is the coding strand and the first codon starts with the first base
5\'-ATGACGTGCTAT-3\'

below is a series of mutations using the coding strand from above write out the new amino acid sequence for each mutation (lower case is mutation)

B. with an insertion: ATGAaCGTGCTAT

C. with a nonsense mutation: ATGACGTGaTAT

D. with a point mutation: ATGACGTGCaAT

i. Using the sequence provided below answer the following questions. A. Write the amino acid sequence for the DNA strand below. Assume this is the coding strand, and the first codon starts with the first base. 5\'-ATG ACGTGCTAT-3\' Below is a series of mutations using the coding strand from above. Write out the new amino acid sequence for each mutation. B. With an insert ATGAACGTGCTAT C. With a nonsense mutation: ATGACGTGATAT D. With a point mutation: ATGACGTGCAAT

Solution

i. Using the sequence provided below answer the following questions.

A. Write the amino acid sequence for the DNA strand below. Assume this is the coding

strand, and the first codon starts with the first base.

5\'-ATG ACGTGCTAT-3\'

For amino acid sequence, first convert DNA sequence to mRNA. And for this , sequence should be same as coding strand except change T to U as thymine is not present in mRNA.
mRNA sequence:

5\'-AUGACGUGCUAU-3\'

Amino acid sequence:

met-thr-cys-tyr


Below is a series of mutations using the coding strand from above. Write out the new

amino acid sequence for each mutation.

B. With an insert
ATGAACGTGCTAT

mRNA seq:

AUGAACGUGCUAU

Amino acid sequence:

met-asn-val-leu

C. With a nonsense mutation: ATGACGTGATAT

mRNA Seq:

AUGACGUGAUAU

Amino acid sequence:

met-thr-stop

D. With a point mutation: ATGACGTGCAAT

mRNA seq:

AUGACGUGCAAU

Amino acid sequence:

met-thr-cys-asn