Using the oxygen dissociation curve determine the saturation

100 80 9 60 E 50 20 ! 10 0 20 40 60 80 100 mm Hg 0 8 12 kPa Partial pressure of O, in blood Using the oxygen dissociation curve, determine the saturation % of hemoglobin in th lungs Blood leaving the lungs carries 20 ml O2 / 100 ml. If there is no Bohr shift, and the oxygen pressure in the tissues is 35 mm Hg, how much oxygen (in ml O2 / 100 ml) is unloaded to the muscles? With the Bohr shift, how much is unloaded (in ml 02/100 ml)? 5.

Solution

The Bohr effect states that the affinity of haemoglobin to oxygen is inversely proportional to acidity and carbon dioxide concentration.

4) In the absence of Bohr effect, 70% oxygen is bound to the haemoglobin. So, 6 ml of O2/100 ml is unloaded to the tissues.

5) In the presence of Bohr effect, the haemoglobin affinity for oxygen decreases to 50%. So, the amount of oxygen unloaded in tissues become 10 ml of O2/100 ml.